Search results for "Leydig Cells"

showing 3 items of 3 documents

Effects of Nandrolone Stimulation on Testosterone Biosynthesis in Leydig Cells

2016

Anabolic androgenic steroids (AAS) are among the drugs most used by athletes for improving physical performance, as well as for aesthetic purposes. A number of papers have showed the side effects of AAS in different organs and tissues. For example, AAS are known to suppress gonadotropin‐releasing hormone, luteinizing hormone, and follicle‐stimulating hormone. This study investigates the effects of nandrolone on testosterone biosynthesis in Leydig cells using various methods, including mass spectrometry, western blotting, confocal microscopy and quantitative real‐time PCR. The results obtained show that testosterone levels increase at a 3.9 μM concentration of nandrolone and return to the ba…

0301 basic medicineEnzymologicMalePhysiologyClinical BiochemistryAndrogenAnabolic Agents; Androgens; Animals; Cell Line; Cell Survival; Dose-Response Relationship Drug; Gene Expression Regulation Enzymologic; Leydig Cells; Male; Nandrolone; Phosphoproteins; Rats; Steroid 17-alpha-Hydroxylase; Testosterone; Physiology; Clinical Biochemistry; Cell BiologyAnabolic AgentsOriginal Research ArticlesNandroloneTestosteroneOriginal Research ArticleTestosteroneAnabolic Agents; Androgens; Animals; Cell Line; Cell Survival; Dose-Response Relationship Drug; Gene Expression Regulation Enzymologic; Leydig Cells; Male; Nandrolone; Phosphoproteins; Rats; Steroid 17-alpha-Hydroxylase; Testosterone; Clinical Biochemistry; Cell Biology; Physiology; Medicine (all)Steroidogenic acute regulatory proteinMedicine (all)Leydig CellsSteroid 17-alpha-HydroxylaseCYP17A1PhosphoproteinAndrogensDrugLuteinizing hormonemedicine.drugAnabolic Agents; Androgens; Animals; Cell Line; Cell Survival; Dose-Response Relationship Drug; Gene Expression Regulation Enzymologic; Leydig Cells; Male; Nandrolone; Phosphoproteins; Rats; Steroid 17-alpha-Hydroxylase; TestosteroneLeydig Cellendocrine systemmedicine.medical_specialtyCell SurvivalBiologyGene Expression Regulation EnzymologicCell LineDose-Response Relationship03 medical and health sciencesDownregulation and upregulationIn vivoInternal medicinemedicineAnimalsDose-Response Relationship DrugAnimalCell BiologyPhosphoproteinsRats030104 developmental biologyEndocrinologyGene Expression RegulationNandroloneAnabolic AgentRatHormone
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Effect of Conjugated Linoleic Acid on Testosterone Levels In Vitro and In Vivo After an Acute Bout of Resistance Exercise

2012

The purposes of the present study were to investigate the effect of conjugated linoleic acid (CLA) supplementation on testosterone levels in vitro on a cell line derived from Leydig cells (R2C) and in vivo in the blood of physically active subjects before and after a resistance exercise bout. In vitro R2C cells were treated with different CLA concentrations (0-30 μM) for 24 and 48 hours. After treatment, supernatant media were tested to determine testosterone secretion. The CLA increased the testosterone secretion only after 48 hours. In vivo, 10 resistance-trained male subjects, in a double-blind placebo-controlled and crossover study design were randomized for 3 weeks of either 6 g·d⁻¹ CL…

AdultMalemedicine.medical_specialtyAnabolismConjugated linoleic acidPhysical Therapy Sports Therapy and RehabilitationCell LineLinoleic Acidchemistry.chemical_compoundSex hormone-binding globulinDouble-Blind MethodIn vivoSex Hormone-Binding GlobulinInternal medicinemedicineAnimalsHumansTestosteroneOrthopedics and Sports MedicineExerciseTestosteroneCross-Over StudiesLeydig cellbiologySettore BIO/16 - Anatomia Umanabusiness.industryfood supplement hormones body composition Leydig cellLeydig CellsResistance TrainingGeneral MedicineCrossover studyRatsmedicine.anatomical_structureEndocrinologychemistryDietary Supplementsbiology.proteinbusinessHormoneJournal of Strength and Conditioning Research
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Differential expression of estrogen receptors (ER?/ER?) in testis of mature and immature pigs

2004

High affinity estrogen receptors (ERs) mediate estrogen action in male reproductive tissues. The objective of the present study was the immunolocalization of estrogen receptor alpha and estrogen receptor beta in immature and mature testes of pig, a species in which the role of estrogens on gonadal function is scarcely known. Testes from 3 and 18 month-old pigs were investigated. Immunohistochemistry was performed on paraffin embedded-tissues using both mouse anti-human monoclonal IgG ERalpha and IgG ERbeta 1 isoform. Western blot analysis demonstrated antibody specificity. ERalpha staining was not observed in immature testes, but it was detected in spermatogonia, spermatocytes and in the mo…

Leydig CellMaleendocrine systemmedicine.medical_specialtySwinemedicine.drug_classSomatic cellBlotting WesternImmunoenzyme TechniqueEstrogen receptorBiologyHeLa CellImmunoenzyme TechniquesWestern blotSpermatocytesInternal medicineTestismedicineAnimalsHumansEstrogen receptorEstrogen Receptor betaEstrogen receptor betaPig developmentmedicine.diagnostic_testAnimalurogenital systemEstrogen Receptor alphaLeydig CellsAntibodies MonoclonalEstrogenAgricultural and Biological Sciences (miscellaneous)SpermatogoniaSpermatocyteBlotEndocrinologyTestiEstrogenImmunohistochemistryAnatomyEstrogen receptor alphahormones hormone substitutes and hormone antagonistsHeLa CellsHumanThe Anatomical Record
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